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1

Microsequence analysis of the N-terminally blocked proteins immobilized on polyvinylidene difluoride membrane by Western blotting

Dr. Hisashi Hirano, Setsuko Komatsu, Hideyuki Kajiwara, Yoshiaki Takagi, Susumu Tsunasawa

Journal:

ELECTROPHORESIS

Year:

1993

Language:

english

File:

PDF, 790 KB

english, 1993

2

Efficient N-terminal peptide sequencing using bis(terpyridine)ruthenium(II) derivatives

Taka-aki Okamura, Norikazu Ueryama, Taku Iwamura, Mana Ikemori, Maki Kaneko, Ken Masui, Hitoshi Yamamoto, Minoru Yamaguchi, Hiroki Kuyama, Eiji Ando, Susumu Tsunasawa

Journal:

Journal of Inorganic Biochemistry

Year:

2003

Language:

english

File:

PDF, 110 KB

english, 2003

3

High-Resolution Crystals of Methionine Aminopeptidase fromPyrococcus furiosusObtained by Water-Mediated Transformation

Tahir H Tahirov, Hideyuki Oki, Tomitake Tsukihara, Kyoko Ogasahara, Katsuhide Yutani, Clare Peters Libeu, Yukiko Izu, Susumu Tsunasawa, Ikunoshin Kato

Journal:

Journal of Structural Biology

Year:

1998

Language:

english

File:

PDF, 107 KB

english, 1998

4

Distinction of Leu and Ile Using a Ruthenium(II) Complex by MALDI-LIFT-TOF/TOF-MS Analysis

Ito, Akihiro, Okamura, Taka-aki, Yamamoto, Hitoshi, Ueyama, Norikazu, Ake, Kojiro, Masui, Ryoji, Kuramitsu, Seiki, Tsunasawa, Susumu

Journal:

Analytical Chemistry

Year:

2005

Language:

english

File:

PDF, 274 KB

english, 2005

5

Messenger RNA Structure Participating in the Initiation of Synthesis of Cucumber Mosaic Virus Coat Protein

HIDAKA, Soh, TSUNASAWA, Susumu, YOON, Joo-Ok, NARITA, Kozo, TAKANAMI, Yoichi, KUBO, Susumu, MIURA, Kin-ichiro

Journal:

Journal of Biochemistry

Year:

1985

Language:

english

File:

PDF, 2.86 MB

english, 1985

6

A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry

Hiroki Kuyama, Keisuke Shima, Kazuhiro Sonomura, Minoru Yamaguchi, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2008

Language:

english

File:

PDF, 640 KB

english, 2008

7

Purification and characterization of a novel solvent-tolerant lipase from Fusarium heterosporum

Yuji Shimada, Chigusa Koga, Akio Sugihara, Toshihiro Nagao, Nobuo Takada, Susumu Tsunasawa, Yoshio Tominaga

Journal:

Journal of Fermentation and Bioengineering

Year:

1993

Language:

english

File:

PDF, 465 KB

english, 1993

8

Crystal structure of methionine aminopeptidase from hyperthermophile, Pyrococcus furiosus

Tahir H Tahirov, Hideyuki Oki, Tomitake Tsukihara, Kyoko Ogasahara, Katsuhide Yutani, Kazuhiro Ogata, Yukiko Izu, Susumu Tsunasawa, Ikunoshin Kato

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 1.11 MB

english, 1998

9

Synthesis and expression of genes encoding tuna, pigeon, and horse cytochromes c in the yeast Saccharomyces cerevisiae

Hickey, David R., Jayaraman, Krishna, Goodhue, Charles T., Shah, Janak, Fingar, Sarah A., Clements, John M., Yumi, Hosokawa, Tsunasawa, Susumu, Sherman, Fred

Journal:

Year:

1991

Language:

english

File:

PDF, 1.10 MB

english, 1991

10

MICROSEQUENCE ANALYSIS OF Nα-BLOCKED PROTEINS ELECTROBLOTTED ONTO AN IMMOBILIZING MATRIX FROM POLYACRYLAMIDE GELS

HIRANO, HISASHI, KOMATSU, SETSUKO, KAJIWARA, HIDEYUKI, TAKAKURA, HIKARU, SAKIYAMA, FUMIO, TSUNASAWA, SUSUMU

Journal:

Analytical Sciences

Year:

1991

Language:

english

File:

PDF, 484 KB

english, 1991

11

Selective isolation of N-terminal peptides from proteins and their de novo sequencing by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry without regard to unblocking or blocking of N-terminal amino acids

Minoru Yamaguchi, Daisuke Nakayama, Keisuke Shima, Hiroki Kuyama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

2008

Language:

english

File:

PDF, 313 KB

english, 2008

12

Determination of the covalent structure of aminoacylase from porcine kidney by ion-spray ionization mass spectrometry

Masaru Miyagi, Hiroshi Ohnogi, Masanori Mitta, Yoshihisa Umeda, Ikunoshin Kato, Fumio Sakiyama, Susumu Tsunasawa

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 255 KB

english, 1992

13

A Peptide that Stimulates Phosphorylation of the Plant Insulin-Binding Protein : Isolation, Primary Structure and cDNA Cloning

Yoshihiro Watanabe, Sergei F. Barbashov, Setsuko Komatsu, Andrew M. Hemmings, Masaru Miyagi, Susumu Tsunasawa, Hisashi Hirano

Journal:

Year:

1994

Language:

english

File:

PDF, 711 KB

english, 1994

14

Characterization of Saccharide Moiety in the Electroplax Sodium Channel.

NAKAYAMA, Hitoshi, YAMAMOTO, Shigeki, HATANAKA, Yasumaru, HACHISU, Tetsuaki, TSUNASAWA, Susumu, SAKIYAMA, Fumio, KANAOKA, Yuichi

Journal:

CHEMICAL & PHARMACEUTICAL BULLETIN

Year:

1992

File:

PDF, 985 KB

1992

15

Sequence analysis of semidwarfism-related proteins in rice

Hisashi Hirano, Setsuko Komatsu, Atsushi Nakamura, Susumu Tsunasawa, Fumio Sakiyama

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 162 KB

english, 1992

16

Electrostatic Stabilization in Methionine Aminopeptidase from Hyperthermophile Pyrococcus f uriosus †

Ogasahara, Kyoko, Lapshina, Elena A., Sakai, Miyo, Izu, Yukiko, Tsunasawa, Susumu, Kato, Ikunoshin, Yutani, Katsuhide

Journal:

Year:

1998

Language:

english

File:

PDF, 107 KB

english, 1998

17

Overproduction of Thermus aquaticus DNA Polymerase and Its Structural Analysis by Ion-Spray Mass Spectrometry

Ishino, Yoshizumi, Ueno, Takashi, Miyagi, Masaru, Uemori, Takashi, Imamura, Mitsuo, Tsunasawa, Susumu, Kato, Ikunoshin

Journal:

Journal of Biochemistry

Year:

1994

Language:

english

File:

PDF, 3.94 MB

english, 1994

18

The Primary Structure of Porcine Aminoacylase 1 Deduced from cDNA Sequence1

Mitta, Masanori, Ohnogi, Hiroshi, Yamamoto, Akira, Kato, Ikunoshin, Sakiyama, Fumio, Tsunasawa, Susumu

Journal:

Journal of Biochemistry

Year:

1992

Language:

english

File:

PDF, 532 KB

english, 1992

19

Reactivity of asparagine residue at the active site of the D105N mutant of fluoroacetate dehalogenase from Moraxella sp. B

Susumu Ichiyama, Tatsuo Kurihara, Yoshifumi Kogure, Susumu Tsunasawa, Haruhiko Kawasaki, Nobuyoshi Esaki

Journal:

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics

Year:

2004

Language:

english

File:

PDF, 263 KB

english, 2004

20

Amino-terminal acetylation of altered form of yeast iso-1-cytochromescin normal andnat1−strains of yeast

Fred Sherman, Richard P. Moerschell, Susumu Tsunasawa, Rolf Sternglanz

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 379 KB

english, 1992

21

Human B-Cell Differentiation Factor Defined by an Anti-Peptide Antibody and Its Possible Role in Autoantibody Production

Toshio Hirano, Tetsuya Taga, Kiyoshi Yasukawa, Koichi Nakajima, Naoko Nakano, Fumihiko Takatsuki, Masatoshi Shimizu, Atsuko Murashima, Susumu Tsunasawa, Fumio Sakiyama and Tadamitsu Kishimoto

Journal:

Proceedings of the National Academy of Sciences

Year:

1987

Language:

english

File:

PDF, 834 KB

english, 1987

22

Preparation of ubiquitin-conjugated proteins using an insect cell-free protein synthesis system

Takashi Suzuki, Toru Ezure, Eiji Ando, Osamu Nishimura, Toshihiko Utsumi, Susumu Tsunasawa

Journal:

Journal of Biotechnology

Year:

2010

Language:

english

File:

PDF, 621 KB

english, 2010

23

The specific isolation of C-terminal peptides of proteins through a transamination reaction and its advantage for introducing functional groups into the peptide

Kazuhiro Sonomura, Hiroki Kuyama, Ei-ichi Matsuo, Susumu Tsunasawa, Osamu Nishimura

Journal:

Rapid Communications in Mass Spectrometry

Year:

2009

Language:

english

File:

PDF, 300 KB

english, 2009

24

Microsequence analysis of N-terminally blocked proteins

Susumu Tsunasawa, Hisashi Hirano

Journal:

The Protein Journal

Year:

1992

Language:

english

File:

PDF, 215 KB

english, 1992

25

The inhibitory properties and primary structure of a novel serine proteinase inhibitor from the fruiting body of the basidiomycete, Lentinus edodes

Shoji Odani, Kei Tominaga, Satomi Kondou, Hiroshi Hori, Takehiko Koide, Saburo Hara, Mamoru Isemura, Susumu Tsunasawa

Journal:

Year:

1999

Language:

english

File:

PDF, 1013 KB

english, 1999

26

Inhibition of Achromobacter Protease I by Lysinal Derivatives

Masaki, Takeharu, Tanaka, Takumi, Tsunasawa, Susumu, Sakiyama, Fumio, Soejima, Masami

Journal:

Bioscience Biotechnology and Biochemistry

Year:

1992

Language:

english

File:

PDF, 791 KB

english, 1992

27

Complete Covalent Structure of Porcine Liver Acylamino Acid-Releasing Enzyme and Identification of Its Active Site Serine Residue

Miyagi, Masaru, Sakiyama, Fumio, Kato, Ikunoshin, Tsunasawa, Susumu

Journal:

Journal of Biochemistry

Year:

1995

Language:

english

File:

PDF, 766 KB

english, 1995

28

Studies on Soybean Trypsin Inhibitors : 2. Amino-Acid Sequence around the Reactive Site of Soybean Trypsin Inhibitor (Kunitz)

Takehiko Koide, Tokuji IKENAKA, Susumu Tsunasawa

Journal:

Year:

1973

Language:

english

File:

PDF, 758 KB

english, 1973

29

Chromatin-related proteins in pluripotent mouse embryonic stem cells are downregulated after removal of leukemia inhibitory factor

Akira Kurisaki, Tatsuo S. Hamazaki, Koji Okabayashi, Tetsuo Iida, Tsutomu Nishine, Ritsu Chonan, Hiroshi Kido, Susumu Tsunasawa, Osamu Nishimura, Makoto Asashima, Hiromu Sugino

Journal:

Biochemical and Biophysical Research Communications

Year:

2005

Language:

english

File:

PDF, 304 KB

english, 2005

30

Expression and activity of a gene encoding rat cytochrome c in the yeast Saccharomyces cerevisiae

Clements, John M., O'Connell, Lisa I., Susumu, Tsunasawa, Sherman, Fred

Journal:

Year:

1989

Language:

english

File:

PDF, 1.44 MB

english, 1989

31

A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus

Takeshi Honda, Yuxin Ni, Schunkichi Hori, Hikaru Takakura, Susumu Tsunasawa, Fumino Sakiyama, Toshio Miwatani

Journal:

FEMS Microbiology Letters

Year:

1989

Language:

english

File:

PDF, 446 KB

english, 1989

32

Two hevein homologs isolated from the seed of Pharbitis nil L. exhibit potent antifungal activity

Ja Choon Koo, So Young Lee, Hyun Jin Chun, Yong Hwa Cheong, Jae Su Choi, Shun-ichiro Kawabata, Masaru Miyagi, Susumu Tsunasawa, Kwon Soo Ha, Dong Won Bae, Chang-deok Han, Bok Luel Lee, Moo Je Cho

Journal:

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology

Year:

1998

Language:

english

File:

PDF, 663 KB

english, 1998

33

[Methods in Enzymology] Posttranslational Modifications Part A Volume 106 || [14] Amino-terminal acetylation of proteins: An overview

Tsunasawa, Susumu

Year:

1984

Language:

english

File:

PDF, 327 KB

english, 1984

34

cDNA Molecular Cloning of Geotrichum candidum Lipase

Shimada, Yuji, Sugihara, Akio, Tominaga, Yoshio, Iizurni, Taro, Susumu, Tsunasawa

Journal:

Journal of Biochemistry

Year:

1989

Language:

english

File:

PDF, 2.19 MB

english, 1989

35

Primary structure of nuclease P1 from Penicillium citrinum

Kazuhiko MAEKAWA, Susumu TSUNASAWA, Gábor DIBÓ, Fumio SAKIYAMA

Journal:

Year:

1991

Language:

english

File:

PDF, 938 KB

english, 1991

36

Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative, clinical isolate of Vibrio parahaemolyticus

Honda, Takeshi, Ni, Yuxin, Hata, Atsuko, Yoh, Myonsun, Miwatani, Toshio, Okamoto, Tadashi, Goshima, Kiyota, Takakura, Hikaru, Tsunasawa, Susumu, Sakiyama, Fumio

Journal:

Canadian Journal of Microbiology

Year:

1990

Language:

english

File:

PDF, 661 KB

english, 1990

37

Direct on-membrane peptide mass fingerprinting with MALDI–MS of tyrosine-phosphorylated proteins detected by immunostaining

Tsuyoshi Nakanishi, Eiji Ando, Masaru Furuta, Susumu Tsunasawa, Osamu Nishimura

Journal:

Journal of Chromatography B

Year:

2007

Language:

english

File:

PDF, 871 KB

english, 2007

38

Strategy for comprehensive identification of human N-myristoylated proteins using an insect cell-free protein synthesis system

Takashi Suzuki, Koko Moriya, Kei Nagatoshi, Yoshinobu Ota, Toru Ezure, Eiji Ando, Susumu Tsunasawa, Toshihiko Utsumi

Journal:

Year:

2010

Language:

english

File:

PDF, 532 KB

english, 2010

39

Primary structure of nuclease P1 from Penicillium citrinum

Kazuhiko MAEKAWA, Susumu TSUNASAWA, Gábor DIBÓ, Fumio SAKIYAMA

Journal:

Year:

1991

Language:

english

File:

PDF, 219 KB

english, 1991

40

A method for N-terminal de novo sequence analysis of proteins by matrix-assisted laser desorption/ionization mass spectrometry

Hiroki Kuyama, Kazuhiro Sonomura, Osamu Nishimura, Susumu Tsunasawa

Journal:

Analytical Biochemistry

Year:

2008

Language:

english

File:

PDF, 258 KB

english, 2008

41

A simple and highly successful C-terminal sequence analysis of proteins by mass spectrometry

Hiroki Kuyama, Keisuke Shima, Kazuhiro Sonomura, Minoru Yamaguchi, Eiji Ando, Osamu Nishimura, Susumu Tsunasawa

Journal:

Year:

2008

File:

PDF, 168 KB

2008

42

The nucleotide sequence of human aminoacylase-1

Masanori Mitta, Ikunoshi Kato, Susumu Tsunasawa

Journal:

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression

Year:

1993

Language:

english

File:

PDF, 214 KB

english, 1993

43

Expression of the human salivary α-amylase gene in yeast and characterization of the secreted protein

Takaaki, Sato, Susumu, Tsunasawa, Yusuke, Nakamura, Mitsuru, Emi, Fumio, Sakiyama, Kenichi, Matsubara

Journal:

Year:

1986

Language:

english

File:

PDF, 1.58 MB

english, 1986

44

Antibodies against 70-kD Heat Shock Cognate Protein Inhibit Mediated Nuclear Import of Karyophilic Proteins

Naoko Imamoto, Yosuke Matsuoka, Toshinao Kurihara, Kenji Kohno, Masaru Miyagi, Fumio Sakiyama, Yoshio Okada, Susumu Tsunasawa and Yoshihiro Yoneda

Journal:

The Journal of Cell Biology

Year:

1992

Language:

english

File:

PDF, 2.89 MB

english, 1992

45

[Methods in Enzymology] Enzyme Structure Part I Volume 91 || [11] Micro-identification of amino-terminal acetylamino acids in proteins by using high-performance liquid chromatography

Tsunasawa, Susumu

Year:

1983

Language:

english

File:

PDF, 951 KB

english, 1983

46

Amino Acid Sequence of Copper, Zinc-Superoxide Dimutase from Spinach Leaves

KITAGAWA, Yasuyuki, TSUNASAWA, Susumu, TANAKA, Nobuo, KATSUBE, Yukiteru, SAKIYAMA, Fumio, ASADA, Kozi

Journal:

Journal of Biochemistry

Year:

1986

Language:

english

File:

PDF, 581 KB

english, 1986

47

Specific isolation of N-terminal fragments from proteins and their high-fidelity de novo sequencing

Minoru Yamaguchi, Takashi Obama, Hiroki Kuyama, Daisuke Nakayama, Eiji Ando, Taka-aki Okamura, Norikazu Ueyama, Takashi Nakazawa, Shigemi Norioka, Osamu Nishimura, Susumu Tsunasawa

Journal:

Rapid Communications in Mass Spectrometry

Year:

2007

Language:

english

File:

PDF, 291 KB

english, 2007

48

Effect of single amino acid substitutions on the protease susceptibility of tryptophan synthase a subunit

Kyoko OGASAHARA, Susumu TSUNASAWA, Yuji SODA, Katsuhide YUTANI, Yoshinobu SUGINO

Journal:

Year:

1985

Language:

english

File:

PDF, 478 KB

english, 1985

49

Complete amino acid sequence of mouse prolactin

Kaoru KOHMOTO, Susumu TSUNASAWA, Fumio SAKIYAMA

Journal:

Year:

1984

Language:

english

File:

PDF, 1.04 MB

english, 1984

50

Multiple γ-glutamylation: A novel type of post-translational modification in a diapausing Artemia cyst protein

Mai Hasegawa, Yuka Ikeda, Hideaki Kanzawa, Mika Sakamoto, Mina Goto, Susumu Tsunasawa, Toshio Uchiumi, Shoji Odani

Journal:

Biochemical and Biophysical Research Communications

Year:

2010

Language:

english

File:

PDF, 519 KB

english, 2010

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